CyFlow Cube 6

CyFlow Cube 6

CyFlow Cube 6 to przenośny, dwu lub jednolaserowy cytometr przepływowy. Jego wyjątkowa konstrukcja, kompaktowa budowa i  dostępność aż 6 parametrów optycznych - Forward Scatter (FSC), Side Scatter (SSC), 4 różne kanały fluorescencji (FL1-FL4) - pozwala na użycie go do większości zastosowań w analizie komórek. Oprogramowanie cytometru pozwala użytkownikowi na wykorzystanie funkcji TVAC (True Volumetric Absolute Counting), która zapewnia całkowite liczenie komórek w określonej objętości. Ta unikatowa technologia nie wymaga dodatkowych odczynników.

Charakterystyka systemu

Charakterystyka systemu

Wysoka wydajność, kompaktowa budowa, wbudowany komputer z 15,4“ monitorem TFT

  • Lasery: niebieski i czerwony
  • Wysoka czułość fluorescencji: ≤ 100 MESF (FITC) | ≤ 50 MESF (PE)
  • Wyjątkowa nanotechnologia: możliwość detekcji cząstek > 50 nm
  • Opcjonalna możliwość instalacji podajnika próbek w postaci probówek i płytek -CyFlow Robby Autoloader
  • TVAC (True Volumetric Absolute Counting) - bezkosztowe, bezpośrednie i niewymagające dodatkowych odczynników liczenie całkowitej liczby komórek
Software

Software

CyView: Intuitive Software for CyFlow Cube 6

The easy-to-use CyView data acquisition and data analysis software is your perfect control tool for operating the CyFlow Cube 6 with maximum efficiency. The Windows™ based CyView software integrates instrument control including acquisition, on- and offline data analysis, on- and offline compensation into a complete software pack- age. predefined and freely adaptable instrument settings and panels facilitate switching between different applications. CyView is dedicated for all applications in immunophenotyping, microbiology, cell cycle analysis, DNA quantification, ploidy analysis, etc. data are stored in FCS flow cytometry standard file format for easy exchange with other analysis software. One of the unique features is the digital on- and offline color crosstalk compensation of the spectral overlap of fluorescence from simultaneously analysed dyes. The N-color software compensation algorithm allows a correction of the crosstalk between any parameters without the need of rerunning a sample.

CyView optimally supports the True Volumetric Absolute Counting feature of the CyFlow Cube 6, displaying particle concentrations for any subsets of cells, even if defined by a gate at a later time after the acquisition.

Specifications

Specifications

Light Sources:

  • Up to 2 simultaneous light sources (laser)
  • o   Blue laser: 50mW @488nm
  • o   Red laser: 25mW @638nm

 

Optics:

  • Modular optical system with selected PMTs with integrated electronic preamplifier for FSC, SSC, FL1-FL4
  • Exchangeable optical filters

 

Flow System:

  • Quartz flow cuvette for laminar sample transport and hydrodynamic focussing
  • True Volumetric Absolute Counting (TVAC) based on mechanical volume measurement
  • Computer controlled precision syringe pump, speed continuously adjustable from 0-20 µl/sec.

 

Software:

  • Windows™ based FCM software CyView for real-time data acquisition, real-time data analysis and real-time data display
  • Easy experimental template set up (configuration files)
  • Flow Cytometry Standard (FCS) data format for storage of original and evaluated data

 

Dimensions:

  • Standalone instrument:
    L 385mm x W 280mm x H 290mm
  • With Autoloading Station: L 745mm 
Upgrades

Upgrades

CyFlow Robby 6 Autoloading Station

General Facts:

  • Protective housing with no light incidence
  • Flexible well/tube measurements

 

Microplates and tubes:

  • 48 or 96-Well-Plates, V, U and flat-bottom are fitting (max.2)
  • Plates meeting the Standards (ANSI/SLAS 1-2004 through 4-2004)
  • 2ml tubes (max. 120)

 

Analysed Volume:

  • Flexible choice of sample volume up to 180 µl for plates and 2000 µl for tubes

 

Speed:

  • Continuously adjustable from 0.2 µl/sec. to 10 µl/sec.
  • ~ 1h for 96-Well-Plate (speed 2 µl/sec., sample volume 20 µl)

 

Software:

  • CyView windows based software

 

Dimensions:

L 360mm x W 370mm x H 270mm 

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